Ancient Dna: Methods And Protocols Link

Over time, DNA strands break into very short fragments, typically between 30 and 100 base pairs.

All work must be done in a dedicated "Clean Lab" with HEPA filtration, positive air pressure, and UV sterilization. Researchers wear full-body suits to prevent shedding their own DNA onto the samples. Ancient DNA: Methods and Protocols

Once extracted, the DNA must be prepared for Next-Generation Sequencing (NGS). Over time, DNA strands break into very short

Software checks for high rates of C-to-T transitions at the ends of DNA fragments. If these "nicks" are present, it’s a signature of authenticity. positive air pressure