C4.zip -

Expression, Purification and Characterization of the Human ... - Nature

: The protein sample is aspirated through the tip, binding to the C4 resin.

: Contaminants are washed away with a dilute acid, such as trifluoroacetic acid (TFA) . c4.zip

A C4 ZipTip is a standard pipette tip containing a microscopic bed of resin fixed at its distal end. Unlike C18 resins, which are used for smaller peptides, the C4 resin is optimized for larger, more hydrophobic molecules like proteins and large polypeptides. Primary Uses in Research Researchers use these tools for two main reasons:

: Best for proteins > 10 kDa and highly hydrophobic peptides. Expression, Purification and Characterization of the Human

: Removing salts, buffers, and detergents that interfere with downstream analysis. For example, researchers use them to clean samples of Trastuzumab probes or mussel biotoxins before testing.

: Enrichment of dilute samples to ensure they are within the detection range of highly sensitive instruments. Role in Mass Spectrometry A C4 ZipTip is a standard pipette tip

: The purified protein is eluted directly onto a target plate using an organic solvent like acetonitrile , where it is mixed with a matrix for analysis. Key Specifications Resin Type : Butyl (C4) silica.