C4zip < FAST 2024 >

Using a high-organic solvent to release the purified protein directly onto a MALDI plate or into a fresh tube. Real-World Application: Mass Spectrometry

Preparing the resin with a wetting solution (e.g., 50:50 acetonitrile in water).

Produced by Millipore , C4 Zip-tips are 10 µL pipette tips containing a specialized C4 (butyl) stationary phase resin. Unlike the more common C18 tips used for peptides, the C4 resin is optimized for larger, more hydrophobic molecules like intact proteins and large polypeptides. Why C4 over C18? Using a high-organic solvent to release the purified

Aspirating and dispensing the protein sample multiple times to maximize binding.

Rinsing with a buffer like 0.1% trifluoroacetic acid (TFA). Unlike the more common C18 tips used for

In recent patent filings and research papers, C4 Zip-tips have been critical for identifying protein isoforms and calculating theoretical masses within 0.5 Da of their primary amino acid sequences. By removing buffers and exchange salts, researchers can achieve the high-resolution mass spectra required for modern drug development and biological research. CN101883788A - 多肽，抗体可变结构域&拮抗剂

While C18 is the "gold standard" for peptide mapping, it can bind proteins so tightly that they become nearly impossible to elute. The shorter carbon chain of the C4 resin offers: Rinsing with a buffer like 0

Essential when working with low-abundance samples.